Effects of zinc (II) -2,4,5-triphenyl-1H-imidazole Complex Compounds on Dengue Virus Serotype 2 Replication

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Effects of zinc (II) -2,4,5-triphenyl-1H-imidazole Complex Compounds on Dengue Virus Serotype 2 Replication

Dengue virus (DENV) is a significant pathogen that appears worldwide as a cause of infectious diseases. DENV is transmitted to humans via female mosquitoes of the Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue fever endemic areas around the world. Dengue fever occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Much effort has been made to prevent and treat DENV infection, and clinical trials of a number of vaccines are currently underway. DENV antiviral testing is an important alternative for drug characterization and development. Complex compounds are formed as a result of the reaction of metal and organic complexes. The complex compound can be used as an anti-inflammatory, antifungal, antibacterial, antiviral antimicrobial. Ion Zn2 ​​+ can be used as an antivirus candidate. The aim of this study was to investigate zinc (II) -2,4,5-triphenyl-1H-imidazole compounds which were further tested for their inhibitory effect on DENV-2 replication in cell culture.

DENV replication was measured by antiviral activity test and cytotoxicity test. The inhibitory activity of zinc (II) -2,4,5-triphenyl-1H-imidazole complex was determined by Viral ToxGloTM Assay. The cytotoxicity of the zinc (II) -2,4,5-triphenyl-1H-imidazole complex was determined by the CellTiter96® AQuoeus test. Meanwhile, to determine the reduction in DENV replication ability due to the effect of these compounds, it can be seen through the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) method.

The inhibitory concentration (IC50) of zinc (II) -2,4,5-triphenyl-1H-imidazole against dengue virus type-2 was 34.42 μg / ml. The cytotoxic concentration (CC50) of the compound against Vero cells was <100 μg / ml. The results of this study indicated the inhibitory activity of investigated serotype 2 antidengue of zinc (II) -2,4,5-triphenyl-1H-imidazole and its high toxicity in Vero cells. Further studies are not needed before investigations of zinc (II) -2,4,5-triphenyl-1H-imidazole can be applied in the treatment of DENV-2 infection.

The stability of the complex is highly dependent on the metal ion and its ligands. As for metal ions (M2 +), Zn (II) is more unstable so that the Zn (II) complex produces more water-soluble ions. This effect causes these complex compounds to become more toxic, because there are more Zn2 + levels in the medium environment so that they can damage the cell walls faster than complex compounds with high stability such as Cu (II), Mn (II), and Co (II).

Based on the results of the amplified DNA electrophoresis, it can be seen that the higher the concentration of zinc (II) -2,4,5-triphenyl-1H-imidazole complexes used to treat DENV-2 in Vero cells shows a thinner band compared to the smallest concentration. . This means that the amount of DENV-2 at high concentrations cannot replicate well.

Author: Teguh Hari Sucipto, et al

Source : news.unair.ac.id

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