{"id":806,"date":"2024-12-24T05:22:21","date_gmt":"2024-12-24T05:22:21","guid":{"rendered":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/?p=806"},"modified":"2025-12-29T04:49:12","modified_gmt":"2025-12-29T04:49:12","slug":"806","status":"publish","type":"post","link":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/2024\/12\/24\/806\/","title":{"rendered":"Potential receptors\u00a0of mosquito-borne arboviruses."},"content":{"rendered":"
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Arboviruses<\/th>\nMolecules<\/th>\nProperties<\/th>\nViruses<\/th>\nCell types<\/th>\nApproaches used to identify\/verify the receptors<\/th>\nInteracted viral components<\/th>\nReferences<\/th>\n<\/tr>\n<\/thead>\n
Mosquito-Borne Flaviviruses<\/strong><\/td>\nDC-SIGN\/L-SIGN\/LSECtin<\/td>\nC-type lectins<\/td>\nDENV, WNV, JEV, ZIKV<\/td>\nMammalian<\/td>\nBinding assays; Immunofluorescence; RNAi assays; Antibody blocking assay; Infection assays in overexpressed non-permissive cell lines; Cryo-EM<\/td>\nprM\/E proteins<\/td>\n(Davis et al., 2006<\/span><\/span><\/a>; Dejnirattisai et al., 2011<\/span><\/span><\/a>; Liu, Ridilla, et al., 2017<\/span><\/span><\/a>; Pokidysheva et al., 2006<\/span><\/span><\/a>; Wang et al., 2016<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
Mannose receptor<\/td>\nDENV<\/td>\nMammalian<\/td>\nBinding Assays; Blot overlay; Immunofluorescence<\/td>\nE protein<\/td>\nMcFadden et al. (2008)<\/span><\/span><\/a><\/td>\n<\/tr>\n
CLEC5A<\/td>\nDENV, JEV, ZIKV<\/td>\nMammalian, Insect<\/td>\nInfection assays in overexpressed non-permissive cell lines; Immunofluorescence; Antibody blocking assay in cell lines and animal models; Flow cytometry<\/td>\nE protein<\/td>\n(Chen et al., 2008<\/span><\/span><\/a>, Chen et al., 2012<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
mosGCTL-1\/3\/7<\/td>\nWNV, DENV, JEV<\/td>\nInsect<\/td>\nGenome-wide RNAi screening; RNAi assays; immunofluorescence; CO-IP<\/td>\nE protein<\/td>\n(Cheng et al., 2010<\/span><\/span><\/a>; Liu et al., 2014<\/span><\/span><\/a>, 2017a<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
TIM receptors<\/td>\nPhosphatidylserine receptors<\/td>\nDENV, WNV, JEV, ZIKV, YFV<\/td>\nMammalian<\/td>\nGain-of-function cDNA screening; Infection assays in overexpressed non-permissive cell; Flow Cytometry; Antibody blocking assay; Immunofluorescence<\/td>\nE protein<\/td>\n(Hamel et al., 2015<\/span><\/span><\/a>; Meertens et al., 2012<\/span><\/span><\/a>; Niu et al., 2018<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
TAM receptors<\/td>\nDENV, ZIKV<\/td>\nMammalian<\/td>\nGain-of-function cDNA screen; RNAi assays; Infection assays in overexpression\/knockout cell lines; Flow Cytometry; Antibody blocking assay; Immunofluorescence<\/td>\nE protein<\/td>\n(Meertens et al., 2012<\/span><\/span><\/a>, 2017<\/span><\/span><\/a>; Richard et al., 2017<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
CD300a<\/td>\nDENV, YFV<\/td>\nMammalian<\/td>\ncDNA library screening; Infection assays in overexpressed cell lines; Flow Cytometry; RNAi assays; Antibody blocking assay<\/td>\nE protein<\/td>\nCarnec et al. (2016)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Integrin \u03b1v\u03b23<\/td>\nIntegrin family<\/td>\nWNV, JEV<\/td>\nMammalian<\/td>\nVOPBA; Antibody blocking assay; RNAi assays; Immunofluorescence; Flow Cytometry<\/td>\nE protein<\/td>\n(Chu and Ng, 2004<\/span><\/span><\/a>; Chu & Ng, 2004<\/span><\/span><\/a>; Fan et al., 2017<\/span><\/span><\/a>; Schmidt et al., 2013<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
Integrin \u03b1v\u03b25<\/td>\nZIKV<\/td>\nMammalian<\/td>\nCRISPR-Cas9 screening; Infection assays in knockout\/overexpression cell lines assays; Immunofluorescence; Antibody blocking assay<\/td>\nViral particle<\/td>\nWang et al. (2020)<\/span><\/span><\/a><\/td>\n<\/tr>\n
HS\/HSPG<\/td>\nGlycosaminoglycans<\/td>\nDENV, JEV, ZIKV, YFV<\/td>\nMammalian<\/td>\nBinding assays; GAG-deficient cell infection assays; In vivo bioluminescence imaging; RNAi assays<\/td>\nE protein<\/td>\n(Gao et al., 2019<\/span><\/span><\/a>; Germi et al., 2002<\/span><\/span><\/a>; Okamoto et al., 2012<\/span><\/span><\/a>; Su et al., 2001<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
37kDa\/67 kDa Laminin receptor<\/td>\nHigh-affinity laminin receptor<\/td>\nDENV, JEV<\/td>\nMammalian, Insect<\/td>\nVOPBA; Binding assays; Liquid chromatography-mass spectrometry (MS); Antibody blocking assay<\/td>\nE protein<\/td>\n(Thepparit & Smith, 2004<\/span><\/span><\/a>; Thongtan et al., 2012<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
GRP78\/HSP70\/90<\/td>\nHeat shock protein family<\/td>\nDENV, ZIKV, JEV<\/td>\nMammalian, Insect<\/td>\nVOPBA; Cell heat shock assay; Antibody blocking assay; RNAi assays; Immunofluorescence; Immunolocalization<\/td>\nE protein<\/td>\n(Das et al., 2009<\/span><\/span><\/a>; Hung et al., 2011<\/span><\/span><\/a>; Pujhari et al., 2019<\/span><\/span><\/a>; Reyes-del Valle et al., 2005<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
NKp44<\/td>\nNatural cytotoxicity receptor family<\/td>\nDENV, WNV<\/td>\nMammalian<\/td>\nElisa; Antibody blocking assay; Flow cytometry; Cytotoxicity assays<\/td>\nE protein<\/td>\nHershkovitz et al. (2009)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Prohibitin-1<\/td>\nProhibitin family<\/td>\nDENV<\/td>\nMammalian, Insect<\/td>\nVOPBA; RNAi assays; Antibody blocking assay; Immunofluorescence<\/td>\nE protein<\/td>\n(Kuadkitkan et al., 2010<\/span><\/span><\/a>; Sharma et al., 2020<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
Claudin-1<\/td>\nClaudin family<\/td>\nDENV<\/td>\nMammalian<\/td>\nYeast two-hybrid (Y2H) screening; Pull-down assays; RNAi assays<\/td>\nprM\/M protein<\/td>\nChe et al. (2013)<\/span><\/span><\/a><\/td>\n<\/tr>\n
nLc4Cer\/GM1a\/GM3<\/td>\nGlycosphingolipid<\/td>\nDENV<\/td>\nMammalian, Insect<\/td>\nTLC\/virus-binding and TLC immunostaining assays; Confocal microscope imaging; MS analysis; ITC; RNAi assays<\/td>\nE protein<\/td>\n(Tantirimudalige et al., 2022<\/span><\/span><\/a>; Wang et al., 2016<\/span><\/span><\/a>; Wichit et al., 2011<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
NCAM1<\/td>\nNCAM family<\/td>\nZIKV<\/td>\nMammalian<\/td>\nProximity labeling using chemical labeled ZIKV particles; LC\u2013MS\/MS analysis; Immunoprecipitation; CRISPR-Cas9 knockout assays; Immunofluorescence<\/td>\nViral particle<\/td>\nSrivastava et al. (2020)<\/span><\/span><\/a><\/td>\n<\/tr>\n
LDLR<\/td>\nLDLR family<\/td>\nJEV<\/td>\nMammalian<\/td>\nRNAi assays; CO-IP; Immunofluorescence; Antibody blocking assay; animal assays<\/td>\nE protein<\/td>\nHuang et al. (2021)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Defensin\/LRP2<\/td>\nAntimicrobial peptides<\/td>\nJEV<\/td>\nInsect<\/td>\nRNAi assays; Immunofluorescence; Antibody blocking assay<\/td>\nE protein<\/td>\nLiu et al. (2020)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Vimentin<\/td>\nIntermediate filament protein<\/td>\nJEV<\/td>\nMammalian<\/td>\nEntry blocking assays; Pull-down<\/td>\nE protein<\/td>\n(Zhang et al., 2024<\/span><\/span><\/a>, Zhang et al., 2024<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
CD4\/CD14<\/td>\nTransmembrane glycoprotein<\/td>\nJEV<\/td>\nMammalian<\/td>\nVOPBA; Binding assays; LC-MS; Antibody blocking assay<\/td>\nE protein<\/td>\n(Thongtan et al., 2012<\/span><\/span><\/a>; Wang et al., 2023<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
PLVAP\/GKN3<\/td>\nTransmembrane glycoprotein\/Gastrokine family<\/td>\nJEV<\/td>\nMammalian<\/td>\nPull down with 2-DE separation and MS; Infection assays in overexpression cell lines; RNAi assays; Antibody blocking assay; Immunofluorescence<\/td>\nE protein<\/td>\nMukherjee et al. (2018)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Mosquito-Borne Alphaviruses<\/strong><\/td>\nLDLRAD3<\/td>\nLDLR family<\/td>\nVEEV<\/td>\nMammalian<\/td>\nCRISPR\u2013Cas9 screening; Infection assays in knockout\/overexpressed cell lines and animal models; Surface plasmon resonance; In vivo imaging; Cryo-EM; Site-directed mutagenesis<\/td>\nE2\u2013E1 heterodimers<\/td>\n(Basore et al., 2021<\/span><\/span><\/a>; Ma et al., 2020<\/span><\/span><\/a>, 2021<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
VLDLR<\/td>\nSFV, EEEV, SINV<\/td>\nMammalian, Insect<\/td>\nCRISPR-Cas9 screening; Infection assays in overexpressed cell lines; Entry blocking assays; Biolayer interferometry binding assays; Confocal microscopy; Cryo-EM<\/td>\nSFV E1 protein,
\nEEEV E2 protein<\/td>\n		(Adams et al., 2024<\/span><\/span><\/a>; Cao et al., 2023<\/span><\/span><\/a>; Clark et al., 2021<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
ApoER2<\/td>\nSFV, EEEV, SINV<\/td>\nMammalian<\/td>\nCRISPR-Cas9 screening; Infection assays in knockout\/overexpressed cell lines; Entry blocking assays; Binding assays; Confocal microscopy<\/td>\nViral particle<\/td>\nClark et al. (2021)<\/span><\/span><\/a><\/td>\n<\/tr>\n
LDLR<\/td>\nEEEV, WEEV, SFV, RRV, GETV, BEBV<\/td>\nMammalian<\/td>\nHost membrane protein expression library screen; Infection assays in knockout\/overexpression cell lines assays; Co-IP; Binding assays; Entry blocking assays<\/td>\nE1 and E2 proteins<\/td>\n(Ma et al., 2024<\/span><\/span><\/a>; Zhai et al., 2024<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
MXRA8<\/td>\nTransmembrane glycoprotein<\/td>\nCHIKV, RRV, ONNV, MAYV, SINV, WEEV, GETV<\/td>\nMammalian, Avian<\/td>\nCRISPR-Cas9 screening; Co-IP; Mass Spectrometry; Binding assays; Infection assays in knockout\/overexpression cell lines and animal models; X-ray Crystallography; Cryo-EM<\/td>\nE1 and E2 proteins<\/td>\n(Basore et al., 2019<\/span><\/span><\/a>; Kim et al., 2020<\/span><\/span><\/a>; Song et al., 2019<\/span><\/span><\/a>; Zhang et al., 2018<\/span><\/span><\/a>; Zimmerman et al., 2023<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
NRAMP\/NRAMP2<\/td>\nNRAMP family<\/td>\nSINV<\/td>\nMammalian, Insect<\/td>\nRNAi assays; Immunofluorescence<\/td>\nViral particle<\/td>\nRose et al. (2011)<\/span><\/span><\/a><\/td>\n<\/tr>\n
CD147<\/td>\nTransmembrane glycoprotein<\/td>\nEEEV, WEEV, SINV, CHIKV, RRV<\/td>\nMammalian<\/td>\nCRISPR-Cas9 screening; CRISPR-Cas9 knockout and complement assays<\/td>\nE2 protein<\/td>\nDe Caluw\u00e9 et al. (2021)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Prohibitin-1<\/td>\nProhibitin family<\/td>\nCHIKV<\/td>\nMammalian<\/td>\n2D-VOPBA; Antibody blocking assay; CO-IP; Immunofluorescence; RNAi assays<\/td>\nE2 protein<\/td>\nWintachai et al. (2012)<\/span><\/span><\/a><\/td>\n<\/tr>\n
37kDa\/67 kDa Laminin receptor<\/td>\nHigh-affinity laminin receptor<\/td>\nSINV, VEEV<\/td>\nMammalian<\/td>\nAntibody blocking assay; Immunoprecipitation; Flow cytometry<\/td>\nE2 protein<\/td>\n(Malygin et al., 2009<\/span><\/span><\/a>; Wang et al., 1992<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
TIM receptors<\/td>\nPhosphatidylserine receptors<\/td>\nCHIKV, RRV, SINV, EEEV<\/td>\nMammalian<\/td>\nInfection assays in overexpressed non-permissive cell lines; Antibody blocking assay; Single particle tracking; Confocal microscope imaging<\/td>\nE2 protein<\/td>\n(Kirui et al., 2021<\/span><\/span><\/a>; Moller-Tank & Maury, 2014<\/span><\/span><\/a>; Pierson et al., 2020<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
TAM receptors<\/td>\nPhosphatidylserine receptors
\nC-type lectins<\/td>\n		RRV, SINV<\/td>\nMammalian<\/td>\nInfection assays in overexpressed non-permissive cell lines; Entry blocking assays; Flow cytometry<\/td>\nViral particle<\/td>\n(Pierson et al., 2020<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
CD300a<\/td>\nSINV<\/td>\nMammalian<\/td>\ncDNA library screening; Infection assays in overexpressed non-permissive cell lines<\/td>\nViral particle<\/td>\nCarnec et al. (2016)<\/span><\/span><\/a><\/td>\n<\/tr>\n
DC-SIGN\/L-SIGN<\/td>\nSINV<\/td>\nMammalian, Insect<\/td>\nFlow cytometry; Immunofluorescence; Infection assays in overexpressed non-permissive cell lines<\/td>\nE1 and E2 proteins<\/td>\nKlimstra et al. (2003)<\/span><\/span><\/a><\/td>\n<\/tr>\n
HS\/HSPG<\/td>\nGlycosaminoglycans<\/td>\nSINV, CHIKV, SFV, RRV, EEEV, VEEV<\/td>\nMammalian<\/td>\nBinding assays; Infection assays in HS\/GAG-deficient cell lines; In vivo imaging<\/td>\nE2 protein<\/td>\n(Gardner et al., 2011<\/span><\/span><\/a>; McAllister et al., 2020<\/span><\/span><\/a>; Ryman et al., 2007<\/span><\/span><\/a>; Smit et al., 2002<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
Integrin \u03b11\u03b21<\/td>\nIntegrin family<\/td>\nRRV<\/td>\nMammalian<\/td>\nAntibody blocking assay; Elisa<\/td>\nE2 protein<\/td>\nLa Linn et al. (2005)<\/span><\/span><\/a><\/td>\n<\/tr>\n
ATP synthase \u03b2 subunit<\/td>\nATPase alpha\/beta chains family<\/td>\nCHIKV<\/td>\nInsect<\/td>\nVOPBA; Mass Spectrometry; Antibody blocking assay; RNAi assays; Confocal microscope imaging<\/td>\nE2 protein<\/td>\nFongsaran et al. (2014)<\/span><\/span><\/a><\/td>\n<\/tr>\n
Mosquito-Borne Bunyaviruses<\/strong><\/td>\nLRP1<\/td>\nLDLR family<\/td>\nRVFV, OROV<\/td>\nMammalian<\/td>\nCRISPR-Cas9 screening; Infection assays in overexpression\/knockout cell line assays; Antibody blocking assay; Biolayer interferometry binding assays; Immunofluorescence<\/td>\nGn glycoproteins<\/td>\n(Ganaie et al., 2021<\/span><\/span><\/a>; Schwarz et al., 2022<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
DC-SIGN\/L-SIGN<\/td>\nC-type lectins<\/td>\nRVFV, LACV, OROV<\/td>\nMammalian<\/td>\nBinding assays; Immunofluorescence; Infection assays in overexpressed non-permissive cell lines; Flow cytometry<\/td>\nGn\/Gc glycoproteins<\/td>\n(L\u00e9ger et al., 2016<\/span><\/span><\/a>; Lozach et al., 2011<\/span><\/span><\/a>)<\/td>\n<\/tr>\n
HS\/HSPG<\/td>\nGlycosaminoglycans<\/td>\nRVFV<\/td>\nMammalian<\/td>\nInfection assays in HS\/GAG-deficient cell lines; Immunofluorescence; Binding assays<\/td>\nViral particle<\/td>\n(de Boer et al., 2012<\/span><\/span><\/a>; Riblett et al., 2016<\/span><\/span><\/a>)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/section>\n","protected":false},"excerpt":{"rendered":"

Arboviruses Molecules Properties Viruses Cell types Approaches used to identify\/verify the receptors Interacted viral components References Mosquito-Borne Flaviviruses DC-SIGN\/L-SIGN\/LSECtin C-type lectins DENV, WNV, JEV, ZIKV Mammalian Binding assays; Immunofluorescence; RNAi assays; Antibody blocking assay; Infection assays in overexpressed non-permissive cell lines; Cryo-EM prM\/E proteins (Davis et al., 2006; Dejnirattisai et al., 2011; Liu, Ridilla, et al., 2017; Pokidysheva et al., 2006; Wang et al.,…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"footnotes":""},"categories":[56],"tags":[59,58],"class_list":["post-806","post","type-post","status-publish","format-standard","hentry","category-kris","tag-mosquito-borne-arboviruses","tag-receptors"],"_links":{"self":[{"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/posts\/806","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/comments?post=806"}],"version-history":[{"count":4,"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/posts\/806\/revisions"}],"predecessor-version":[{"id":810,"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/posts\/806\/revisions\/810"}],"wp:attachment":[{"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/media?parent=806"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/categories?post=806"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/itd.unair.ac.id\/itd\/entomology2\/wp-json\/wp\/v2\/tags?post=806"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}