A Preliminary Study of Dengue Virus Serotype in Aedes Mosquitoes in Surabaya in January 2020

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A Preliminary Study of Dengue Virus Serotype in Aedes Mosquitoes in Surabaya in January 2020

Aedes sp. is a mosquito that can act as a vector of Dengue Hemorrhagic Fever (DHF). This mosquito contains the dengue virus (DENV) which comes from the blood of people with dengue fever. Dengue virus consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3 and DENV-4. Aedes aegypti mosquito is the main vector causing DHF, while Aedes albopictus is a secondary vector causing DHF. The adult Aedes albopictus mosquito is characterized by a basic black color with white patches on the body and legs. These white spots are actually scales attached to the outside of the mosquito’s body. On the back chest (mesonotum) of Aedes aegypti there are white curved lines left and right, and in the middle there are two longitudinal white lines that resemble a harp instrument while Aedes albopictus has only one white line.

In this report, we observe the presence of Dengue virus in Aedes albopictus from the area of ​​the Institute of Tropical Disease, Universitas Airlangga. Our observations started from mosquito identification, RNA preparation and extraction, RNA purity testing with Nanodrop Spectrophotometer, converting RNA to cDNA with the Reverse Transcriptase enzyme, DNA amplification with the Polymerase Chain Reaction method using Lanciotti et al 1992 primers, electrophoresis, and DNA visualization with UV transilluminators. The mosquitoes that were collected were identified as female Aedes albopictus mosquitoes based on microscopic observation, there was a thick white line on the mesonotum without two curved lines and the antennae were like threads with short hair. DNA visualization amplified by UV transilluminators showed that there was a band containing dengue virus serotype 2 (DENV-2). This is consistent with previous research in 2013, DENV-3 emerged when DENV-1 became the most cases during 2008-2013. It has been predicted that DENV-3 will emerge next year. In fact, it turned out that DENV-3 did become an epidemic in 2013-2016. This suggests the possibility that our newly discovered DENV-2 could become an epidemic in the future. So that this research can be used as an effort to help health workers to monitor and get ready for the eradication of the outbreak in the future.

Authors: Teguh Hari Sucipto, Shifa Fauziyah, Aulia Azzahra, Lucky Vera Oktavia, Muhammad Fariz Naviyanto, et al.

Source : news.unair.ac.id

The complete article can be seen at the following link: http://www.envirobiotechjournals.com/article_abstract.php?aid=10885&iid=320&jid=3

(Preliminary Study of Dengue Virus Serotype on Aedes Mosquitoes in Endemic Area, Surabaya, Indonesia, January 2020)

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