{:en}Institute of Tropical Disease (ITD) Universitas Airlangga (UNAIR) continuously hold workshops for students, researchers, and practitioners. Last week, was Gastroenteritis Laboratory and Hepatitis Laboratory held a workshop, Basic and Molecular Technique on Bacteriology: Sequence Analysis of Carbapenemase Genes in Bacteria. The activity was held on Wednesday, October 16 to Friday, October 18.
Interviewed during the event, the workshop chairman, Dr. Dadik Raharjo, drh., M. Kes., explained that the activity was focused on learning basic molecular biology techniques to observe the presence of specific genes in bacteria. The aim is to detect the danger of these bacteria associated with pathogenicity and their resistance to antibiotics. Some ITD researchers were also invited to support the workshops.
“We invited some of the researchers to provide material such as Prof. Retno Handayani, dr., MS., Ph.D and myself. While practical activities in the laboratory are carried out by supervision and other researchers from the Gastroenteritis Laboratory and the Hepatitis Laboratory. On that occasion, we would like to share experiences in identifying bacteria with carbapenemase gene, “said the lecturer in Faculty of Veterinary Medicine.
Carbapenemase is a type of β-lactamase enzyme with the ability to hydrolyze the antibiotics of penicillin, cephalosporin, monobactam, and carbapenem classes. Bacteria that produce β-lactamase can cause infections that are difficult to treat because they make many antibiotics with active β-lactam ingredients ineffective.
“This workshop was held in collaboration with the Gastroenteritis Laboratory working in bacterial culture, resistance testing, and gene detection in bacteria by PCR technique while the Hepatitis Laboratory led by Prof. Soetjipto, dr. MS., Ph.D, has extensive experience in molecular biology with one of its technologies which is sequencing. This is done so that participants can get a complete insight, “explained Dr. Dadik.
About seven workshop participants were guided from the most basic techniques to the sequencing stage along with the analysis of sequencing results. The workshop is expected to improve the ability of participants to apply the PCR method and sequencing as part of technology in molecular biology. Besides, Dr. Dadik hoped that there will be more academic interested in this field.
“Because, molecular biology is related in every basic life and has broad functions, including infectious diseases, vaccine development, antibiotic production, food products, bioenergy production, protein production, cloning technology, CRISPR-based chromosome editing technology, and halal products,” stated Dr. Dadik (*)
Author: Nabila Amelia Source : news.unair.ac.id{:}{:id}Lembaga Penyakit Tropis (ITD) Universitas Airlangga (UNAIR) kian gencar untuk mengadakan workshop bagi mahasiswa, peneliti, dan praktisi. Pekan lalu, giliran Laboratorium Gastroenteritis bersama Laboratorium Hepatitis menggelar workshop bertajuk Basic and Molecular Technique on Bacteriology: Sequence Analysis of Carbapenemase Genes in Bacteria. Kegiatan itu berlangsung sejak hari Rabu (16/10) sampai dengan Jumat (18/10).
Ditemui di sela kegiatan, ketua workshop, Dr. Dadik Raharjo, drh., M.Kes., menjelaskan bahwa kegiatan itu difokuskan untuk mempelajari teknik dasar molekuler biologi guna mengamati keberadaan gen spesifik pada bakteri. Tujuannya adalah mendeteksi bahaya dari bakteri tersebut terkait dengan pathogenitas maupun resistensinya terhadap antibiotik. Sejumlah peneliti ITD pun dihadirkan guna mendukung kelancaran workshop.
“Beberapa peneliti yang kami undang untuk memberikan materi adalah Prof. Retno Handayani, dr., MS., Ph.D juga saya sendiri. Sementara kegiatan praktek di laboratorium dilaksanakan oleh supervisi serta peneliti lainnya dari Laboratorium Gastroenteritis dan Laboratorium Hepatitis. Pada kesempatan itu, kami ingin berbagi pengalaman dalam mengidentifikasi bakteri yang memiliki gen carbapenemase,” tutur dosen Fakultas Kedokteran Hewan itu.
Carbapenemase adalah jenis enzim β-laktamase dengan kemampuan menghidrolisis antibiotik golongan penisilin, sefalosporin, monobaktam, dan karbapenem. Bakteri yang memproduksi β-laktamase dapat mengakibatkan infeksi yang sukar diobati karena membuat banyak antibiotik yang mempunyai bahan aktif β-laktam menjadi tidak efektif.
“Workshop ini diadakan atas kerjasama dari Laboratorium Gastroenteritis yang berkecimpung di kultur bakteri, uji resistensi, dan deteksi gen pada bakteri dengan teknik PCR. Sedangkan Laboratorium Hepatitis yang dipimpin Prof. Soetjipto, dr. MS., Ph.D, telah memiliki pengalaman luas dalam molekuler biologi dengan salah satu teknologinya yakni sequencing. Hal ini dilakukan supaya para peserta bisa memperoleh wawasan yang lengkap,” urai Dr. Dadik.
Sebanyak tujuh orang peserta workshop dibimbing mulai dari teknik yang paling dasar sampai tahap sequencing beserta analisis hasil sequencing. Melalui workshop tersebut, diharapkan dapat meningkatkan kemampuan peserta dalam mengaplikasikan metode PCR dan sequencing sebagai bagian dari teknologi dalam molekuler biologi semakin meningkat. Di samping itu, Dr. Dadik berharap agar peminat bidang ini kian bertambah.
“Sebab, molekuler biologi itu hampir ada di setiap basic kehidupan dan memiliki fungsi yang luas. Di antaranya dalam hal penyakit infeksi, perkembangan vaksin, produksi antibiotik, produk pangan, produksi bioenergi, produksi protein, teknologi kloning, teknologi editing kromosom berbasis CRISPR, dan produk halal,” pungkas Dr. Dadik. (*)
Penulis : Nabila Amelia Sumber : news.unair.ac.id{:}