On August 1– 3, 2023, Institute of Tropical Disease in collaboration with Research Centre for Global Emerging and Reemerging Infectious Disease (RC-GERID) was successfully hosting the 7th MCC workshop. This annual workshop adopted a topic on “Real-time PCR and ELISA: Molecular and Serological Approach in Biomedical Research”. In total, 15 enthusiastic participants from various background joined this workshop, which made the event more intense and fruitful.
The first day of the workshop involved lectures from the experts and hands-on DNA isolation as well as preparation for real-time PCR. We invited Dr. Ni Luh Ayu Megasari to deliver a lecture on “Sample Preparation for real-time PCR & Enzyme-linked Immunosorbent Assay”. We also invited product specialist from PT Merck Chemicals and Life Sciences and PT Biotek Prima Indoplus to present about basic technique of Enzyme-linked Immunosorbent Assay and Primer & Probe Design as well as Real-Time PCR. During the lecture a pleasant discussion between the speaker and participants was apparent. The workshop facilitated participants to experience hands-on DNA isolation and preparation for real-time PCR afterwards. During the hands-on, each group of participants were personally guided by instructors, who were the researchers of ITD.
The second day of the workshop was fully laboratory work on Enzyme-linked Immunosorbent Assay. The product specialist from PT Merck Chemicals and Life Sciences, Taufik Indramawan, demonstrated the step-by-step ELISA protocols as well as Multiplex ELISA to the participants. The participants also enjoyed the laboratory work for ELISA as discussions happened with excitement.
In the last day, participants were joining the data analysis for ELISA and realtime PCR. They were guided to read the results of realtime PCR machine and interpret the result correctly. During this session, product specialist from PT Biotek Prima Indoplus, Audi Putra Santosa, mentored the participants to analysis and interpret the results. Meanwhile, for data analysis of ELISA results were guided by Taufik from PT. Merck Chemicals. The organizing committee received good feedback from the participants. Most of the participants stated that they will definitely join the next MCC workshop.