Viral Diarrhea

BACKGROUND

Viral Diarrhea study group has been built since August 2015, as a new research laboratory at the Institute of Tropical Disease (ITD) UNAIR. We conduct various research projects that focus on viral diarrhea including Rotavirus and Norovirus in pediatric and adults patients infected with acute and chronic gastroenteritis of various hospitals and health centers in Indonesia. In recent research, we are collaborating with some researchers from Kobe University. Our concern is to do molecular epidemiology of rotavirus and norovirus strain in Indonesia with collecting stool samples from some areas in Indonesia, such as Java, Nusa Tenggara, Papua, Maluku, Sumatera and etc. Our goal is to understand the prevalency and molecular study of rotavirus and norovirus spreading in Indonesia. Until to date, we realize that there is not many

RESEARCH ACTIVITIES

RESEARCHERS

1. Prof.Soetjipto, dr., MS., Ph.D
2. Prof. Maria Inge Lusida, dr., M.Kes., Ph.D., Sp. MK (K).
3. Takako Utsumi, Ph.D
4. Dr.Juniastuti,dr.,M.Kes
5. Laura Navika Yamani, S.Si, M.Si, Ph.D.
6. Rury Mega Wahyuni, DVM., M.Si
7. Zayyin Dinana, DVM

LABORATORY ACTIVITIES

1. Rotavirus

   This study focused on examination of rotavirus in pediatric and adults patients who are infected with acute and chronic gastroenteritis of various hospitals in Indonesia, especially in the vicinity of Surabaya. In addition, we also collect published papers yet related with viral diarrhea research, especially norovirus study in Indonesia. Moreover, we do research the whole genome of rotavirus and norovirus performed by Next Generation sequencing to examine of virus reassortment.We expect that our result can provide information to us and clinician about the occurence of diarrhea caused by virus, especially rotavirus and also study about the characterization of the virus. Investigation performed in Viral Diarrhea laboratory, among others: • Immunochromatography test • Conventional PCR and Real-Time PCR • Direct Sequencing • Genotyping and phylogenetic tree analysis samples from children who had been vaccinated with the rotavirus vaccine to evaluate the effectiveness of the vaccine.

2. Norovirus

   This research was conducted by collecting stool samples from pediatric and adults patients who are infected with acute and chronic gastroenteritis of various hospitals in Indonesia, especially in the vicinity of Surabaya. As Norovirus infection control in accordance to the data of previous studies in several countries, we are also doing research on stool samples from healthy people. Norovirus research results on this project is an initial information in Indonesia.

OUTPUT

1. Soetjipto, Takako Utsumi, Maria Inge lucida, Rury Mega Wahuni, Zayyin Dinana, Juniastuti, Laura Navika Yamani, Subijanto Marto Sudarmo, Alpha Fardah Athiyyah, Andi Darma, Chieko Matsui, Deng Lin, Yen Hai Doan, Kazuhiko Katayama, and Ikuo Shoji. A preliminary survey of norovirus infections Among children in Surabaya, Indonesia. International seminar of the Global Strategy to Combat Emerging Infectious Diseases in Borderless Era (GSEID). 2016
2. Zayyin Dinana, Takako Utsumi, Rury Mega Wahuni, Laura Navika Yamani, Chieko Matsui, Deng Lin, Maria Inge lucida, Soetjipto, Yen Hai Doan, Kazuhiko Katayama, and Ikuo Shoji. Occurrence of norovirus infection is an asymptomatic population in Indonesia. International seminar of the Global Strategy to Combat Emerging Infectious Diseases in Borderless Era (GSEID). 2016